QUANTITATIVE EXTRACTION OF HAMSTER LIVER LIPID AND CHOLESTEROL WITH SUPERCRITICAL CARBON-DIOXIDE

Authors
KAHLON TS CHOW FI SAYRE RN
Citation
Ts. Kahlon et al., QUANTITATIVE EXTRACTION OF HAMSTER LIVER LIPID AND CHOLESTEROL WITH SUPERCRITICAL CARBON-DIOXIDE, Journal of the American Oil Chemists' Society, 73(10), 1996, pp. 1341-1342
Citations number
15
Categorie Soggetti
Food Science & Tenology","Chemistry Applied
Journal title
Journal of the American Oil Chemists' Society → ACNP
ISSN journal
0003021X
Volume
73
Issue
10
Year of publication
1996
Pages
1341 - 1342
Database
ISI
SICI code
0003-021X(1996)73:10<1341:QEOHLL>2.0.ZU;2-N
Abstract
A quantitative method for liver lipid and cholesterol extraction with supercritical CO2 and ethanol entrainer (SCE) is reported and compared with the Folch (chloroform/methanol) procedure. Mean values for lipid and cholesterol in hamster livers (n=48) were similar between the SCE and Folch methods. Correlation coefficients between the two methods w ere 0.9866 for total lipid and 0.9546 for cholesterol. Similar mean va lues and high correlations between the two methods validate the SCE pr ocedure as a precise alternative method ior quantitative liver lipid e xtractions. The SCE method also reduces the use of hazardous solvents.