LARGE-SCALE PROCEDURE FOR FRACTIONATION OF ALBUMINS AND GLOBULINS FROM PEA-SEEDS

The buffer extractable proteins of pea, albumins and globulins, were s uccessively extracted in a large amount at a pilot scale. In order to preserve as much as possible the native structure of proteins, a selec tive solubilization step procedure was performed. Firstly, albumins we re extracted with acetate buffer and secondly globulins with phosphate buffer of high ionic strength. Each extract was desalted by diafiltra tion without protein precipitation. From 15 kg of flour, 380 g of albu min fraction and 1000 g of globulin fraction were obtained with a prot ein content of 86.0% and 90.7% of dry matter respectively. The charact erisation of albumin and globulin fractions by electrophoresis, ultrac entrifugation and anion-exchange chromatography, showed that the cross -contamination of these two fractions was minimal.