SEMIPREPARATIVE ISOLATION OF PHOSPHOPEPTIDES DERIVED FROM BOVINE CASEIN AND DEPHOSPHORYLATION OF CASEIN PHOSPHOPEPTIDES

Semi-preparative isolation of casein phosphopeptides (CPP) from a tryp tic hydrolysate of bovine casein was performed applying a three-step p rocedure consisting of solid phase extraction, reversed phase HPLC and ion exchange chromatography. Dephosphorylation of CPP was achieved us ing immobilized alkaline phosphatase. The purified phosphopeptides and their dephosphorylated forms obtained by these methods are suitable f or comparative studies on biological activities, especially mineral bi nding and immunmodulation.