COSOLUTE EFFECT ON CRYSTALLIZATION OF 2 DINUCLEOTIDE COMPLEXES OF BOVINE SEMINAL RIBONUCLEASE FROM CONCENTRATED SALT-SOLUTIONS

Authors
SICA F ADINOLFI S VITAGLIANO L ZAGARI A CAPASSO S MAZZARELLA L
Citation
F. Sica et al., COSOLUTE EFFECT ON CRYSTALLIZATION OF 2 DINUCLEOTIDE COMPLEXES OF BOVINE SEMINAL RIBONUCLEASE FROM CONCENTRATED SALT-SOLUTIONS, Journal of crystal growth, 168(1-4), 1996, pp. 192-197
Citations number
17
Categorie Soggetti
Crystallography
Journal title
Journal of crystal growth → ACNP
ISSN journal
00220248
Volume
168
Issue
1-4
Year of publication
1996
Pages
192 - 197
Database
ISI
SICI code
0022-0248(1996)168:1-4<192:CEOCO2>2.0.ZU;2-#
Abstract
Two complexes of bovine seminal ribonuclease with dinucleotides, uridy lyl(2'-5')adenosine (UpA) and 2'-deoxycytidylyl(3'-5')-2'-deoxyadenosi ne (d(CpA)), were crystallized under unusual conditions involving a li quid-liquid phase separation. This phenomenon was induced by adding sm all aliquots of organic cosolutes to highly concentrated ammonium sulf ate solutions. The liquid-liquid interface acts as a source of nucleat ion centers for growth of large crystals. Among the cosolutes tested i n these salt-mediated crystallizations, 2-methyl-2,4-pentanediol was f ound to be the most efficient.