G. Marquezruiz et al., QUANTITATION OF OXIDIZED TRIGLYCERIDE MONOMERS AND DIMERS AS AN USEFUL MEASUREMENT FOR EARLY AND ADVANCED STAGES OF OXIDATION, Grasas y aceites, 47(1-2), 1996, pp. 48-53
Quantitation of oxidized triglyceride monomers and dimers is reported
as a good measurement for early and advanced stages of oxidation. Appl
icability of this approach to follow-up oxidation was tested in sample
s of trilinolein and methyl linoleate stored at either room temperatur
e or 60 degrees C for different periods of time. Oxidized monomers, di
mers and polymers were determined in 50 mg-samples by adding monostear
in as internal standard and applying a combination of adsorption chrom
atography, using silica cartridges, followed by high-performance size-
exclusion chromatography. Additionally, peroxide values and tocopherol
contents were measured. Results showed that a significant rise of dim
eric compounds denoted the end of the induction period while oxidized
monomers were the only group of compounds showing a progressive increa
se during the early stages of oxidation.