Rb. Rastogi et Re. Levin, METABOLIC-ACTIVATION AND INACTIVATION OF METANIL-YELLOW AND ORANGE-IIIN THE SALMONELLA-TYPHIMURIUM HIS(-) REVERSION ASSAY, Journal of food protection, 59(3), 1996, pp. 244-248
Among a variety of experimental protocols used, the combined use of 0.
5% dextrose in bottom agar and 1 mu mol of flavin mononucleotide (FMN)
in preincubation mixtures without fraction S9 mix resulted in the hig
hest level of induced Salmonella typhimurium his(-) reversions with bo
th dyes metanil yellow and orange II with strain TA100. Strain TA98 yi
elded notably lower levels of reversions under the same conditions. Th
e presence of uninduced hamster Liver S9 fraction resulted in a weak m
utagenic response while Aroclor 1254-induced rat liver S9 fraction res
ulted in the complete absence of mutagenicity with both strains and wi
th both dyes.