The family of small heat shock proteins is the more variable among the
highly conserved superfamily of heat shock proteins (HSP). Using a me
tabolic labeling procedure with tissue explants, we have detected in c
hickens a new member of the small HSP family with an apparent molecula
r weight of 29-kDa. This protein was induced in broiler chickens' hear
t muscle and lungs following an in vivo heat stress. The 29-kDa band a
ppears after 3 h of heat stress, much later than the induction of HSP
90, HSP 70, and HSP 27. The late onset of induction suggests that HSP
29 plays a more specific role of a ''second stage defense protein''.