G. Sreeramulu et Nk. Singh, HIGH M(R) GLUTENIN SUBUNITS OF INDIAN WHEAT CULTIVARS - ASSOCIATION OF SUBUNITS-5-1RS WHEAT-RYE TRANSLOCATION(10 WITH THE 1BL), Journal of cereal science, 20(3), 1994, pp. 217-225
The seed proteins of lid commercially-released Indian wheat cultivars
were fractionated using sodium dodecyl sulphate polyacrylamide gel ele
ctrophoresis (SDS-PAGE) to determine their high M(r) glutenin subunit
compositions. Amongst the cultivars, three alleles were identified for
the Glu-Al locus (subunits 1, 2 and the null phenotype), eight allel
es for the Glu-Bl locus (7, 7+8, 7+9, 6+8, 20, 13+16, 17+18 and a new
allele) and two for the Glu-Dl locus (2+12 and 5+10). Nine of the cult
ivars were heterogeneous and possessed two or more 'biotypes' with res
pect to high M(r) subunits. The cultivars were also analysed for the p
resence of the IBL/IRS wheat-rye translocation by SDS-PAGE of unreduce
d prolamins and hybridisation of DNA dot blots with a rye-telomere spe
cific repetitive DNA probe, pAW-161. Both methods revealed that the ma
jority of newly-released Indian wheat cultivars carry this translocati
on, thus confirming the agronomic superiority of these lines. While mo
st of the normal wheat cultivars possessed high M(r) subunits 2+12, 14
of the 18 translocation cultivars had the allelic subunits 5+10, even
though no selection was made for these subunits during the breeding p
rocess. This suggests that the subunits 5+10 may play a compensating r
ole for the loss of dough strength associated with the IBL/IRS translo
cation.