Ms. Kuk et Rj. Hron, SUPERCRITICAL CARBON-DIOXIDE EXTRACTION OF COTTONSEED WITH COSOLVENTS, Journal of the American Oil Chemists' Society, 71(12), 1994, pp. 1353-1356
Extraction of cottonseed lipids with supercritical carbon dioxide (SC-
CO2) was conducted with and without a co-solvent, ethanol or 2-propano
l (IPA). At 7000 psi and 80 degrees C, the reduced pressure, temperatu
re and density of SC-CO2 was at 6.5, 1.17 and 1.85, respectively; the
specific gravity was 0.87. Under these conditions, CO2 is denser than
most liquid extraction agents such as hexane, ethanol and IPA. The ext
raction of cottonseed with SC-CO2 gave a yield of more than 30% (moist
ure-free basis). This is comparable to yields obtained by the more com
monly used solvent, hexane. The crude cottonseed oil extracted by SC-C
O2 was visually lighter than refined cottonseed oil. This was substant
iated by colorimetric measurements. No gossypol was detected in the cr
ude oil. However, crude oil extracted by SC-CO2, to which less than 5%
of ethanol or IPA as co solvent was added, contained ca. 200 ppm of g
ossypol, resulting in the typical dark color of cottonseed crude oil w
ith gossypol. CO2, extracted a small amount of cottonseed phosphatides
, about one-third of that extracted by pure ethanol, IPA or hexane. A
second extraction with 100% ethanol or IPA after the initial SC-CO2 ex
traction produced a water soluble lipid fraction that contained a sign
ificant amount of gossypol, ranging between 1500 and 5000 ppm. Because
pure gossypol is practically insoluble in water, this fraction is bel
ieved to be made up of gossypol complexed with polysaccharides and pho
sphatides.