SIMULTANEOUS DETERMINATION OF MALTOSE AND GLUCOSE USING A DUAL-ELECTRODE FLOW-INJECTION SYSTEM

A dual-electrode system consisting of a glucose oxidase (GOD) electrod e and an amyloglucosidase/glucose oxidase (A/G) electrode in an FIA ma nifold was constructed to determine maltose and glucose simultaneously in their mixture. Highly active enzyme membranes were acquired. The l inear range of the system was 0.3-30 mmol/litre of sugars. The Variati on coefficients were 3.2% for the GOD electrode and 1-6% for the A/G e lectrode in measuring the mixture of glucose and maltose. The results made by the dual-electrode system agreed well with those made by Fehli ng titration. During 20 days' determination, the activities of the enz yme electrodes showed no decay.