D. Madigan et al., RAPID-DETERMINATION OF 4-VINYL GUAIACOL AND FERULIC ACID IN BEERS ANDWORTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of the American Society of Brewing Chemists, 52(4), 1994, pp. 152-155
Sensitive methods for the determination of ferulic acid and 4-vinyl gu
aiacol in beers and worts are described. Samples were analyzed by dire
ct-injection high-performance liquid chromatography using an octadecyl
silica column. Detection was by either fluorescence monitoring (for 4
-vinyl guaiacol alone) or electrochemical detection (for simultaneous
determination of ferulic acid and 4-vinyl guaiacol). The methods were
applied mainly to the analysis of production beers but were used also
to monitor the decarboxylation of ferulic acid to 4-vinyl guaiacol in
wort during a small-scale fermentation deliberately contaminated with
a wild yeast strain.