Semen was collected for 4 consecutive d individually from experimental
broiler breeder males that had not been massaged for 7 d. The semen w
as mixed and diluted with the Beltsville Poultry Semen Extender with d
imethylsulfoxide (DMSO) as cryoprotectant and cryopreserved. After tha
wing of the semen, hens were inseminated and fertility over 1 wk after
a single insemination with the cryopreserved semen was determined. Th
e overall fertility rate in this experiment (83 to 93%) was high, comp
ared with the generally reported fertility rate of frozen-thawed fowl
semen. The fertility rate of the semen collected on Days 3 and 4 was s
ignificantly higher than that of semen collected on Day 1 or 2, indica
ting that frequent collection over the 4-d period enhances the fertili
ty rate of the semen.