This paper deal with the main aspects of the analysis of oxysterols. T
his analytical problem is quite new and many questions involved are st
ill controversial. Consequently, many methodologies exist that still n
eed improvement and standarization. Therefore, it is interesting to di
scuss the analytical methodologies proposed by different authors for t
he extraction, purification and determination (gas chromatography and
high performance liquid chromatography) of oxysterols.