Tcrm. Deoliveira et al., A SIMPLE AND RAPID ANTIBODY-CAPTURE ELISA FOR THE DETECTION OF STAPHYLOCOCCAL-ENTEROTOXIN-A IN FOOD INCLUDING A SIMPLE EXTRACTION STEP, International journal of food science & technology, 29(5), 1994, pp. 563-573
A sensitive and precise ELISA for the detection of staphylococcal ente
rotoxin A in food has been developed, using specific IgG anti-toxin an
tibodies purified from immunized rabbits. An antibody capture format w
as found to be the most useful following cross-reaction and sensitivit
y studies. The ELISA was used to investigate concentration of staphylo
coccal enterotoxin A from food samples by polyethylene glycol dialysis
. The procedure was shown to be inefficient and highly variable, as we
ll as time-consuming. Direct analysis of aqueous food extracts without
a concentration step was found to be possible. With an assay time of
140 min, the ELISA detection limit was 12.5 pg of toxin (corresponding
to a sensitivity of 0.5 ng g(-1) of food sample).