PREDICTION OF CLOTTING TIME FOR MILK COAGULATION BY MIXTURES OF PROTEOLYTIC-ENZYMES

Kinetics of milk coagulation by three proteolytic enzymes, chymosin, b ovine pepsin and neutrase, at varying concentrations, were monitored b y thrombelastography. Clotting time for milk coagulation by chymosin w as linear (r(2) = 0.997) with the reciprocal of enzyme concentration, whereas the best equation for pepsin (r(2) = 0.997) and neutrase (r(2) = 0.999) was the one relating the reciprocal of clotting time to enzy me concentration. When the effect of mixtures of enzymes on milk coagu lation was studied, the best-fitting regression was that of the recipr ocal of clotting time on concentration of enzymes for both chymosin-pe psin(r(2) = 0.998) and chymosin-neutrase (r(2) = 0.998) mixtures.