PROGENY ANALYSIS OF TOBACCO THAT EXPRESS A MAMMALIAN DELTA-9 DESATURASE

Authors
GRAYBURN WS HILDEBRAND DF
Citation
Ws. Grayburn et Df. Hildebrand, PROGENY ANALYSIS OF TOBACCO THAT EXPRESS A MAMMALIAN DELTA-9 DESATURASE, Journal of the American Oil Chemists' Society, 72(3), 1995, pp. 317-321
Citations number
13
Categorie Soggetti
Food Science & Tenology","Chemistry Applied
Journal title
Journal of the American Oil Chemists' Society → ACNP
ISSN journal
0003021X
Volume
72
Issue
3
Year of publication
1995
Pages
317 - 321
Database
ISI
SICI code
0003-021X(1995)72:3<317:PAOTTE>2.0.ZU;2-N
Abstract
Various tissues of Nicotiana tabacum plants transformed with a rat ste aryl-CoA desaturase gene were analyzed for lipid composition. Gas chro matographic analysis showed a decrease in saturated 16 and 18 carbon f atty acids in leaves and roots of several progeny. Polymerase chain re action analysis of total DNA from leaves indicated the presence of the desaturase gene in kanamycin-resistant progeny with altered fatty aci d composition. Western immunoblot analysis confirmed the presence of i ntroduced desaturase protein. Data on the inheritance of kanamycin res istance is presented.