Ws. Grayburn et Df. Hildebrand, PROGENY ANALYSIS OF TOBACCO THAT EXPRESS A MAMMALIAN DELTA-9 DESATURASE, Journal of the American Oil Chemists' Society, 72(3), 1995, pp. 317-321
Various tissues of Nicotiana tabacum plants transformed with a rat ste
aryl-CoA desaturase gene were analyzed for lipid composition. Gas chro
matographic analysis showed a decrease in saturated 16 and 18 carbon f
atty acids in leaves and roots of several progeny. Polymerase chain re
action analysis of total DNA from leaves indicated the presence of the
desaturase gene in kanamycin-resistant progeny with altered fatty aci
d composition. Western immunoblot analysis confirmed the presence of i
ntroduced desaturase protein. Data on the inheritance of kanamycin res
istance is presented.