CHOLESTEROL - DETERMINATION IN MUSCLE, AD IPOSE-TISSUE, AND IN OFFAL BY HPLC

Authors
ARNETH W
Citation
W. Arneth, CHOLESTEROL - DETERMINATION IN MUSCLE, AD IPOSE-TISSUE, AND IN OFFAL BY HPLC, Die Fleischwirtschaft, 75(2), 1995, pp. 185-187
Citations number
NO
Categorie Soggetti
Food Science & Tenology
Journal title
Die Fleischwirtschaft → ACNP
ISSN journal
0015363X
Volume
75
Issue
2
Year of publication
1995
Pages
185 - 187
Database
ISI
SICI code
0015-363X(1995)75:2<185:C-DIMA>2.0.ZU;2-D
Abstract
A method for the determination of cholesterol in animal tissues by HPL C is described. The extraction of cholesterol(esters) from the sample is achieved by a mixture of hexane and isopropanol (3:2), which has be en proven as the best procedure with the help of radioactive tracers. In an aliquot of the extract a methanolysis of the cholesterolesters i s made; interfering substances are removed on a silicagel-column, whic h was standardized in its activity by addition of 10% water. Cholester ol is eluted from the silica-column by dichloromethan. The chromatogra phic analysis is performed isocratically on a RP18-column by a mixture of acetonitrile and isopropanol (80:20) measuring at a wavelength of 210 nm. During the entire analysis stigmasterol as internal standard i s used.