A survey of microbiological and physico-chemical parameters of minced
lamb packed under modified atmosphere and sold by five major supermark
ets nation-wide in the U.K. was made. Samples were analysed on the pur
chase and on the expiry day. Lactic acid bacteria (Carnobacterium pisc
icola, Carnobacterium divergens, Leuconostoc mesenteroides subsp. mese
nteroides, Lactococcus raffinolactis), Brochothrix thermosphacta, pseu
domonads (Pseudomonas fragi, Pseudomonas fluorescens, Pseudomonas lund
ensis), Enterobacteriaceae and yeats, given in order of numerical impo
rtance, were members of the microbial associations on the expiry day.
Of the physico-chemical attributes pH decreased, glucose, glucose 6-ph
osphate, gluconate were catabolised, and, L-, D-lactic and acetic acid
and ammonia were produced. Dominance of lactic acid bacteria was refl
ected in a decrease in pH, production of D-lactic and acetic acid and,
following depletion of the carbohydrates, an oxidation of lactate iso
mers to acetate. When B. thermosphacta was dominant these changes were
not detected to the same extent. When a large population of pseudomon
ads was present oxidation of glucose to gluconate occurred.