STUDY OF NEUTRAL LIPIDS OF LUPINUS-MUTABILIS MEAL AND ISOLATES

Two types of protein isolates have been obtained from defatted Lupinus mutabilis meal. The isolates, MA and MB, were obtained by alkaline ex traction with 0.2% NaOH and 0.25% sodium bisulfite, respectively, foll owed by precipitation at the isoelectric point (pH 4.8). Total associa ted lipids were extracted with 86% ethanol. Neutral lipids were separa ted in a Florisil column. The lipids in the isolates were similar to t hose found in the original meal. The following types of compounds were separated, identified, and quantitated: hydrocarbons, waxes, methyl e sters, triacylglycerols, free fatty acids, diacylglycerols, and free s terols.