HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ANALYSIS OF PEANUT PHOSPHOLIPIDS .1. INJECTION SYSTEM FOR SIMULTANEOUS CONCENTRATION AND SEPARATION OF PHOSPHOLIPIDS
Ja. Singleton et Lf. Stikeleather, HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ANALYSIS OF PEANUT PHOSPHOLIPIDS .1. INJECTION SYSTEM FOR SIMULTANEOUS CONCENTRATION AND SEPARATION OF PHOSPHOLIPIDS, Journal of the American Oil Chemists' Society, 72(4), 1995, pp. 481-483
This paper discusses the details of a high-performance liquid chromato
graphy method for the simultaneous concentration and separation of pho
spholipids or other trace compounds by direct oil injection using two
different solvent systems. The system equilibrates and concentrates ph
ospholipids on a silica column using hexane. At the same time, an anal
ytical column is equilibrating and separating phospholipids using two
binary solvent mixtures. This system eliminates a preconcentration ste
p previously accomplished by solid-phase extraction, open-column chrom
atography, and other previously used methodology. Other advantages inc
lude: a 40% reduction in analysis time, elimination of a second transf
er of labile compounds, decreased solvent use, and a simpler array of
solvents to separate phospholipids. The method described has broader a
pplications, such as trace organic compounds in water supplies, and tr
ace metals with appropriate modifications for the particular analysis.