HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ANALYSIS OF PEANUT PHOSPHOLIPIDS .1. INJECTION SYSTEM FOR SIMULTANEOUS CONCENTRATION AND SEPARATION OF PHOSPHOLIPIDS

This paper discusses the details of a high-performance liquid chromato graphy method for the simultaneous concentration and separation of pho spholipids or other trace compounds by direct oil injection using two different solvent systems. The system equilibrates and concentrates ph ospholipids on a silica column using hexane. At the same time, an anal ytical column is equilibrating and separating phospholipids using two binary solvent mixtures. This system eliminates a preconcentration ste p previously accomplished by solid-phase extraction, open-column chrom atography, and other previously used methodology. Other advantages inc lude: a 40% reduction in analysis time, elimination of a second transf er of labile compounds, decreased solvent use, and a simpler array of solvents to separate phospholipids. The method described has broader a pplications, such as trace organic compounds in water supplies, and tr ace metals with appropriate modifications for the particular analysis.