DETERMINATION OF IPRODIONE AND ITS METABO LITE IN FOODS BY HPLC

An HPLC method for determination of iprodione and its metabolite in ag ricultural products was developed, The residues were extracted from ve getables and fruit with acetone or from cereal and beans with acetonit rile, and then re-extracted into ethyl acetate. The extracts were clea ned up by Florisil column chromatography. The Florisil column was wash ed with 50 ml of 50% ether in n-hexane, and iprodione and its metaboli te were eluted with 50 ml of 15% acetone in n-hexane. Recoveries of ip rodione and its metabolite added to samples at 0.5 ppm ranged from 70. 3 to 105.0% (except from tea), and the detection limits were 0.02 ppm. Tea gave low recoveries because iprodione and its metabolite were sca rcely extracted from tea leaves with hot water. Iprodione dissolved in methanol gradually changed to its metabolite during storage at room t emperature. Therefore the standard solution should be freshly prepared as occasion demands.