Porcine M. longissimus dorsi from 12 carcasses were used to study the
effect of ante mortem exercise treatment, time of boning and different
ageing periods on tenderness and tenderisation in pork. The pigs were
randomly allocated into three groups, of which the pigs (in two of th
em) were exposed to work on a treadmill: (1) 30 min rest after work, t
hen slaughtered; (2) slaughtered without rest after work; and (3) cont
rols, which were not exposed to work before slaughter. At 1 and 6 h po
st-stunning, samples from right side of each carcass were excised and
immediately chilled in icewater until 24 h post-stunning. The left sid
e loins were used as controls and were chilled on the carcasses at 12-
14 degrees C for 90 min and then at 2 degrees C before boning at 24 h
post-stunning. The effect of ageing, 0 or 7 days at 2 degrees C, was s
tudied. No parameter in this study was significantly affected by ante
mortem treatment. WE shear force decreased significantly with increasi
ng boning time and was correlated to the degree of muscle shortening i
n unaged excised samples. Muscle shortening for the 1 h excised cuts s
howed an average value of 27.4%, while an average value of only 6.1% w
as obtained for 6 h excised cuts. Ageing for 7 days resulted in only m
inor (7.3%) tenderness improvement for the I h excised cuts as compare
d to the 24.5% decrease in WE shear force obtained from the 6 h excise
d, less cold-shortened cuts. These differences in ageing rate, however
, did not result in detectable changes in the degradation profile of m
yofibrillar proteins as analysed by SDS-PAGE.