The anti-sarcoma response of three B complex recombinant haplotypes B-
R1(F24-G23), B-R2(F2-G23), and B-R3(F2-G23) was investigated. In a pre
liminary experiment, one male heterozygous for the B-R1 recombinant ha
plotype and another heterozygous for the B-R2 recombinant haplotype we
re each mated to females, some of which carried the respective recombi
nant. The anti-sarcoma response of progeny carrying the B-R1 recombina
nt differed significantly from that of progeny carrying the B-R2 recom
binant. Subsequently, each of the three recombinant haplotypes was pla
ced on each of four B haplotype complex backgrounds, and compared to B
-G and B-L/B-F region controls on the same background haplotype. For e
ach recombinant, significant differences in tumor growth were found be
tween the recombinant and B-L/B-F control chickens on either one, two,
or three of the four genetic backgrounds tested. For each recombinant
, no differences were found between chickens carrying the recombinant
and B-G region controls, which is further evidence that the gene(s) co
ntrolling Rous sarcoma growth lies in or near the B-L/B-F chromosomal
region. Moreover, although the B-R2 and B-R3 recombinants appear to be
identical serologically, they differed significantly in tumor growth
suggesting that the two haplotypes are genetically different.