THE MICRO WATER 1,2-DICHLOROETHANE INTERFACE AS A TRANSDUCER FOR CREATININE ASSAY

Facilitated ammonium ion transfer reactions, using the crown ether dib enzo-18-crown-6, at a single microhole supported water/1,2-dichloroeth ane interface were used as the transducer to detect the biomolecule cr eatinine. The ammonium ion was produced during the enzymatic action of creatinine deiminase on creatinine. A prototype sensing device incorp orating a Teflon gas permeable membrane, to exclude possible interfere nts, yielded a linear amperometric response to added creatinine from 2 0 muM to 1 mM. This range is sufficient for that required for the dete rmination of creatinine in serum.