Md. Osborne et Hh. Girault, THE MICRO WATER 1,2-DICHLOROETHANE INTERFACE AS A TRANSDUCER FOR CREATININE ASSAY, Mikrochimica acta, 117(3-4), 1995, pp. 175-185
Facilitated ammonium ion transfer reactions, using the crown ether dib
enzo-18-crown-6, at a single microhole supported water/1,2-dichloroeth
ane interface were used as the transducer to detect the biomolecule cr
eatinine. The ammonium ion was produced during the enzymatic action of
creatinine deiminase on creatinine. A prototype sensing device incorp
orating a Teflon gas permeable membrane, to exclude possible interfere
nts, yielded a linear amperometric response to added creatinine from 2
0 muM to 1 mM. This range is sufficient for that required for the dete
rmination of creatinine in serum.