Cj. Shieh et al., 4-FACTOR RESPONSE-SURFACE OPTIMIZATION OF THE ENZYMATIC MODIFICATION OF TRIOLEIN TO STRUCTURED LIPIDS, Journal of the American Oil Chemists' Society, 72(6), 1995, pp. 619-623
The ability of an immobilized lipase to modify the fatty acid composit
ion of (88.8% C-18:1, 4.3% C-16:0, 3.1% C-18:0, and 3.8% C-18:2 as det
ermined by gas chromatography, and approximately 90% triolein) in hexa
ne by incorporation of a medium-chain fatty acid, capric acid (C-10),
to form structured triacylglycerol was studied. Response surface metho
dology was used to evaluate the effect of synthesis variables, such as
reaction time (12-36 h), temperature (25-65 degrees C), molar substra
te ratio of capric acid to triolein (2:1-6:1), and enzyme amount (10-3
0% wt% of triacylglycerol), on the yield of structured lipid. Optimiza
tion of the transesterification was attempted to obtain maximum yield
of structured lipid while using the minimum molar substrate ratio and
enzyme amount as much as possible. Computer-generated contour plot int
erpretation revealed that a relatively high molar substrate ratio (6:1
) combined with low enzyme amount (10%) after 30 h of reaction at 25 d
egrees C gave optimum incorporation of capric acid. A total yield for
combined mono- and dicaproolein of up to 100% was obtained.