CARICA-PAPAYA LATEX LIPASE - SN-3 STEREOSELECTIVITY OR SHORT-CHAIN SELECTIVITY - MODEL CHIRAL TRIGLYCERIDES ARE REMOVING THE AMBIGUITY

Short-chain fatty acids are usually located at position sn-3 in natura l triglycerides, particulary in dairy fats. As a result, it is extreme ly difficult to differentiate between sn-3 stereospecificity and short -chain typoselectivity in many lipases and acyltransferases that perfo rm in this way. This ambiguity can be removed through successive use o f a chiral triglyceride with a short fatty acid in position sn-1 and o f its racemic in controlled hydrolysis reactions. After checking that the proposed method effectively confirmed the type of activity of cont rol biocatalysts (Candida cylindracea nonspecific lipase and Mucor mie hei 1-3 regiospecific lipase), we confirmed that Carica papaya latex h as a strict sn-3 stereospecificity.