M. Hosokawa et al., PHOSPHOLIPASE A(2)-MEDIATED SYNTHESIS OF PHOSPHATIDYLETHANOLAMINE CONTAINING HIGHLY UNSATURATED FATTY-ACIDS, International journal of food science & technology, 29(6), 1994, pp. 721-725
Phosphatidylethanolamine (PE), which contains highly unsaturated fatty
acid (HUFA) specifically in sn-2 position, was synthesized by porcine
pancreatic phospholipase A(2)-catalysed esterification of lysophospha
tidylethanolamine with HUFA. The use of glycerol containing only a lit
tle water as solvent was the important key to this reaction. A 27% yie
ld of up to 94.5% sn-2 eicosapentaenoic acid (EPA)-containing PE was o
btained using >99% EPA as a substrate HUFA.