DETERMINATION AND SURVEY OF STARTING MATE RIALS AND SUBSIDIARY COLORSIN NEW COCCINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A method for determination of the starting materials, [7-hydroxy-1,3,6 -naphthalenesulfonic acid (TS), G-salt (GS), R-salt (RS), Schaeffer's salt (SS) and naphthionic acid(NA)] and subsidiary colors, [ponceau 6R (P6R), amaranth (R-2) and fast red E (FRE)] in new coccine (R-102) wa s developed by use of HPLC. The following conditions were used for ana lysis: column, L-column ODS (4.6 mm i.d. x 250 mm); eluent, gradient e lution system of 0.02 M ammonium acetate and acetonitrile (hold at 0.0 2 M ammonium acetate for 5 min, then 0 similar to 30% acetonitrile in 45 min); detection for TS, GS, RS, SS and NA at 238 nm and subsidiary colors at 510 nm. Recoveries of each impurity from R-102 averaged 99.1 similar to 103.5%. Ten samples of commercial R-102 were analyzed by t he present HPLC method and the impurites were as follows: TS, 0.005 si milar to 0.044%; GS, 0.044 similar to 0.284%; NA, 0.013 similar to 0.1 96%: RS, N.D.; SS N.D.; P6R, 0.008 similar to 0.169%; R-2, N.D. simila r to 0.279%; FRE, 0.007 similar to 0.100%. The detection limit was 0.0 01% for each impurity.