Rj. Millischer et al., EVALUATION OF THE GENOTOXICITY POTENTIAL AND CHRONIC INHALATION TOXICITY OF 1,1-DICHLORO-1-FLUOROETHANE (HCFC-141B), Food and chemical toxicology, 33(6), 1995, pp. 491-500
A battery of in vitro and in vivo tests were conducted on HCFC-141b as
a vapour. Bacterial gene mutation assays with Escherichia coli and Sa
lmonella typhimurium were negative in all tester strains. In vitro chr
omosomal aberration assays were positive on CHO cells but negative on
human lymphocytes. Moreover, HCFC-141b was negative in vivo in a mouse
micronucleus inhalation assay. On the basis of these data and previou
sly reported genotoxicity testing, HCFC-141b is considered non-genotox
ic. Groups of 80 male and 80 female Sprague-Dawley rats were exposed,
by inhalation (6 hr/day, 5 days/wk) to vapours of HCFC-141b for 104 wk
at target concentrations of 0 (control), 1500, 5000 and 20,000 ppm (i
ncreased from 15,000 ppm after 17 wk of exposure). No exposure-related
effects of toxicological significance were noted with respect to surv
ival, clinical signs, ophthalmoscopy, haematology, clinical chemistry,
urinalysis or organ weight analysis. Reduced food intake and body wei
ght gain were noted in both sexes of the 15,000 ppm group during the f
irst 16 wk; thereafter, body weight gains in all groups were similar a
lthough the intergroup differences in body weight remained evident. Re
duced food intake persisted in both sexes through wk 52 and in females
during the second year of exposure. Treatment-related effects on macr
oscopic pathology were confined to increased incidences of testicular
masses and altered appearance. Microscopic pathology examinations conf
irmed the testes as the target organ with findings of increased incide
nces of benign interstitial cell tumours and hyperplasia at 5000 and 2
0,000 ppm. The no-observable-adverse-effect level (NOAEL) was 1500 ppm
. The testicular changes at high exposure levels were considered to be
due to a change of the senile hormonal imbalance in geriatric rats an
d of little significance for the assessment of human health effects.