ANTIOXIDANT ACTIVITY OF PEPTIDE FRACTIONS OF CAPELIN PROTEIN HYDROLYSATES

Four peptide fractions were separated from protein hydrolysates of cap elin (Mallotus villosus) using Sephadex G-10 gel filtration column chr omatography. Antioxidant activity of each fraction was determined in a beta-carotene-linoleate model system. One isolated fraction possessed a notable antioxidant activity, another two had a weak efficacy while the fourth exerted a prooxidant effect. Two-dimensional thin layer ch romatography (TLC) of isolated fractions gave spots with both antioxid ant and prooxidant activities. Two prooxidant compounds were separated from the hydrolysate by preparative TLC using silica gel plates. One compound exhibited a maximum absorption at 254 nm while the other abso rbed at 260 nm. Copyright (C) 1996 Elsevier Science Ltd.