ASSESSMENT OF LIPOXYGENASE ACTIVITY IN SE ED EXTRACTS FROM 35 PLANT-SPECIES

Lipoxyganase activity in 35 seed extracts was determined an the basis of hydroperoxide synthesis using linoleic acid as substrate. The resul ts referring to the extracted protein content show that several specie s of Vigna (V. unguiculata, V. radiata and V. mungo) and one of Trifol ium exhibit stronger lipoxygenase activity than soybean enzyme extract s. The pH-activity relationship was also established for 4 very active samples. GCMS analysis revealed equal amounts of 9- and 13 hydroperox ides of linoleic acid In these seed extracts, indicating no enzyme pos itional specificity.