RAPID DNA PURIFICATION FOR HAL GENE PCR DIAGNOSIS IN PORCINE TISSUES AND EXTENSION TO OTHER MEAT SPECIES

Two different halothane (Hal) gene polymerase chain reaction (PCR) tes ts were applied to genomic DNA extracted from por cine blood, semen, m uscle and fat tissues by a rapid and simple Chelex-100 based method. O ne of the PCR procedure is designed from the ryanodine receptor coding sequence to produce a 81 base pair (bp) fragment, while the other is designed from pig intron sequences to produce a 659 bp fragment. Oligo nucleotide primers derived from the coding sequence were also used for other meat species. Amplification products obtained from porcine, bov ine, ovine, equine and deer genomic DNA were successfully digested wit h Hha I restriction enzyme to produce the same electrophoretic pattern as in the normal homozygous (NN) pig. No PCR products could be amplif ied from chicken and turkey DNA. Copyright (C) 1996 Elsevier Science L td.