The effect of dietary alpha-tocopheryl acetate supplementation on the
uptake of alpha-tocopherol in ewe plasma, lamb plasma, milk, organs an
d muscles was investigated. The oxidative stability and colour in fres
h M. longissimus dorsi and frozen M. longissimus dorsi, M. psoas major
and M. gluteus medius were also investigated. Ewes (n = 12) were sele
cted and scanned to assess pregnancy, They were divided into two group
s (n = 6). The control group was fed a diet containing 20 mg alpha-toc
opheryl acetate/kg feed/day and the supplemented group fed a diet cont
aining 1000 mg alpha-tocopheryl acetate/kg feed/day, for 9 weeks ante-
parturition and 3 weeks post-parturition. The lambs were weaned at 3 w
eeks and fed supplemented or basal feed for 10 weeks before slaughter.
Plasma alpha-tocopherol increased significantly (p < 0.01) in ewes in
the 9 weeks ante-parturition, and lamb plasma taken just before slaug
hter was significantly (p < 0.01) higher for the supplemented group th
an the basal group, following 13 weeks of supplementation. Milk alpha-
tocopherol levels were significantly (p < 0.01) higher from ewes fed t
he supplemented diet at parturition and for the three weeks of supplem
entation post-parturition (p < 0.05). Supplementation increased the al
pha-tocopherol levels in all tissues sampled. The alpha-tocopherol con
centrations in M. longissimus dorsi and M. psoas major were also deter
mined after frozen storage at -20 degrees C for 34 weeks. Frozen stora
ge resulted in a significant (p < 0.01) reduction in mean alpha-tocoph
erol levels for M. longissimus dorsi but not M. psoas major. Dietary s
upplementation with alpha-tocopheryl acetate significantly (p < 0.05)
increased the oxidative stability of lamb muscle. Surface colour (Hunt
er L, a, b) was found to be negatively correlated with metmyoglobin co
ntent. Supplementation reduced surface discolouration in refrigerated
display under fluorescent light over a 6-7 day storage period. The eff
ect It as more pronounced in frozen displayed muscles than in freshly
displayed samples. Copyright (C) 1996 Elsevier Science Ltd.