EVALUATION OF MICELLAR LIQUID-CHROMATOGRAPHY AND CAPILLARY ZONE ELECTROPHORESIS FOR DOPE CONTROL IN SPORT

Micellar liquid chromatography (MLC) and capillary zone electrophoresi s (CZE) have been evaluated for the analysis of twelve banned drugs in sport including diuretics and beta-blockers. In MLC, a sodium dodecyl sulphate aqueous solution has been used as mobile phase using an octad ecylsilica column. In CZE, a pH 8 buffer solution and a silica capilla ry have been employed. Parameters of retention and efficiency have bee n compared. Limits of detection with UV detection at 254 nm and relati ve standard deviations for atenolol, furosemide, nadolol, spironolacto ne and triamterene were established and compared in both techniques. E xamples of direct urine injection into the separation systems are pres ented. Drugs overlapping in MLC are well resolved in CZE, while the op posite is true for a limited number of drugs. Some interferences from urine may arise in CZE. The selectivity of analysis would be greatly e nhanced by using both techniques, which require only filtration as pre -treatment.