Jm. Vadillo et al., EVALUATION OF MICELLAR LIQUID-CHROMATOGRAPHY AND CAPILLARY ZONE ELECTROPHORESIS FOR DOPE CONTROL IN SPORT, Mikrochimica acta, 118(3-4), 1995, pp. 273-282
Micellar liquid chromatography (MLC) and capillary zone electrophoresi
s (CZE) have been evaluated for the analysis of twelve banned drugs in
sport including diuretics and beta-blockers. In MLC, a sodium dodecyl
sulphate aqueous solution has been used as mobile phase using an octad
ecylsilica column. In CZE, a pH 8 buffer solution and a silica capilla
ry have been employed. Parameters of retention and efficiency have bee
n compared. Limits of detection with UV detection at 254 nm and relati
ve standard deviations for atenolol, furosemide, nadolol, spironolacto
ne and triamterene were established and compared in both techniques. E
xamples of direct urine injection into the separation systems are pres
ented. Drugs overlapping in MLC are well resolved in CZE, while the op
posite is true for a limited number of drugs. Some interferences from
urine may arise in CZE. The selectivity of analysis would be greatly e
nhanced by using both techniques, which require only filtration as pre
-treatment.