IN-VITRO FRUCTOOLIGOSACCHARIDE UTILIZATION AND INHIBITION OF SALMONELLA SPP. BY SELECTED BACTERIAL

In vitro experiments were conducted to determine: 1) inhibitory capaci ties of potential direct-fed microbial bacteria against Salmonella ser otypes; and 2) the ability of Bifidobacterium bifidum, Enterococcus fa ecium, Lactobacillus casei, Lactococcus lactis, Pediococcus sp., and S almonella spp. to grow in media containing fructooligosaccharides (FOS -50(R) or FOS pure formulation) as the only carbohydrate source. Thirt een bacteria (two strains of Bacillus coagulans, Bacillus licheniformi s, Bacillus subtilis, B. bifidum, E. faecium, two strains of Lactobaci llus acidophilus, L. casei, Pediococcus sp., Propionibacterium acidopr opionici, P. jensenii, and Propionibacterium sp.) were tested for inhi bition of six Salmonella serotypes (S, california, S, enteritidis, S. heidelberg, S. mission, S. senftenberg, and S. typhimurium) using a sp ot-the-lawn technique. Bifidobacterium bifidum, E. faecium, all lactob acilli, and Pediococcus sp. clearly inhibited growth of all Salmonella serotypes. In the growth experiments, E. faecium, L. lactis, and Pedi ococcus sp. grew in media with either FOS-50(R) or the pure formulatio n of FOS as the sole carbohydrate source. All tested Salmonella seroty pes utilized FOS-50(R) for growth; however growth varied among the ser otypes. In contrast, none of the Salmonella serotypes grew in media co ntaining the pure formulation of FOS as the only carbohydrate source.