M. Bulte et P. Jakob, THE USE OF A PCR-GENERATED INVA PROBE FOR THE DETECTION OF SALMONELLASPP IN ARTIFICIALLY AND NATURALLY CONTAMINATED FOODS, International journal of food microbiology, 26(3), 1995, pp. 335-344
Part of the invasion A gene (invA) of salmonellae (Rahn et al., 1992)
was amplified and labelled simultaneously with digoxigenin by the poly
merase chain reaction (PCR). This was used as gene probe for a colony
hybridization assay which included nitrocellulose filter incubation on
modified Rambach agar. 312 Salmonella and 268 non-Salmonella strains
were hybridized with the invA probe. No false-negative or false-positi
ve results were obtained. In 11 beef samples, which had been contamina
ted artificially with Salmonella, the test strain was recovered quanti
tatively with the invA probe. Salmonellae could be detected in 29 samp
les of 104 further foods of animal origin by means of the gene probe a
ssay in contrast to 27 samples which were positive by the standard met
hod. The invA probe assay allows for the quantitative estimation of Sa
lmonella in fresh meat samples within 48 h. However, with frozen sampl
es a pre-enrichment step is necessary.