STM images of 10.7 nm dendrimer microspheres were obtained in order to
provide a model study of STM imaging of large insulating objects. Neg
ative contrast in these images, observed before only on biological sam
ples,demonstrates that this phenomenon cannot be explained by the biol
ogical specificity of a specimen. Large insulating objects may appear
as depressions on the constant current STM image due to the interplay
of electronic and mechanical interactions between the tip, the substra
te and the adsorbate. Utilization of macroscopic elasticity constants
in a model of three elastic spheres gives a good correspondence with t
he reported experimental data. This model can be modified in future in
order to obtain microscopic mechanical properties of nm-size objects
in STM experiments.