Internal mold infection levels for microwave yellow popcorn, nonmicrow
ave white popcorn, nonmicrowave yellow popcorn and specialty popcorn w
ere determined by direct plating of kernels on Dichloran-rose bengal-c
hloramphenicol agar (DRBC), Dichloran-18% glycerol agar (DG-18), Asper
gillus flavus/parasiticus agar (AFPA) and Czapek's iprodione agar (CZI
D). The total percentage of mold-infected kernels was low in microwave
yellow popcorn (5.9%) and nonmicrowave yellow popcorn (7.3%), but som
ewhat higher in specialty popcorn (13.6%) and nonmicrowave white popco
rn (15.1%). Of the molds found, Fusarium species predominated in the m
icrowave yellow popcorn (54.2%) and nonmicrowave white popcorn (66.2%)
, whereas Aspergillus species predominated in the nonmicrowave yellow
popcorn (43.8%) and specialty popcorn (52.9%). Of the Fusarium species
isolated, F. moniliforme was the predominant species in all types of
popcorn including microwave yellow (70.4%), nonmicrowave white (70.4%)
, nonmicrowave yellow (72.3%) and specialty popcorn (85.7%). Other Fus
arium species found were F. proliferatum, F. semitectum, F. subglutina
ns, F. anthophilum, and F. graminearum. The presence of the Aspergillu
s flavus-parasiticus group was negligible in the popcorn samples evalu
ated. No significant differences were found among media in enumerating
total mold infection levels. DG-18 was found to be effective in enume
rating Aspergillus species, but ineffective in enumerating Fusarium sp
ecies from popcorn. No significant differences were found among other
media in enumerating Aspergillus and Fusarium species.