A. Zampelas et al., EFFECTS OF DIETARY FATTY-ACID COMPOSITION ON BASAL AND HORMONE-STIMULATED HEPATIC LIPOGENESIS AND ON CIRCULATING LIPIDS IN THE RAT, British Journal of Nutrition, 74(3), 1995, pp. 381-392
Thirty male rats were randomly assigned to one of three dietary groups
in which the source of dietary fat was either a mixed oil, maize oil
or fish oil. Effects of dietary fatty acid composition on in vitro rat
es of [U-C-14]glucose incorporation into hepatic total lipids and into
hepatic triacylglycerol were measured under basal, insulin (4 nM)-, g
astric inhibitory polypeptide (GIP; 6 nM)- and insulin + GIP (4 nM + 6
nM)-stimulated conditions. Effects of the three diets on postprandial
plasma triacylglycerol, cholesterol, insulin and GIP concentrations w
ere also measured. The fish-oil diet decreased rates of basal glucose
incorporation into hepatic total lipids (P < 0.05) and hepatic triacyl
glycerol (P < 0.01) compared with the mixed-oil diet. The presence of
insulin+GIP in the incubation medium stimulated glucose incorporation
into hepatic total Lipids in the maize-oil (P < 0.01) and fish-oil gro
ups (P < 0.05), as well as into hepatic triacylglycerol in the maize-o
il group (P < 0.005). In addition, the fish-oil diet decreased postpra
ndial plasma triacylglycerol levels compared with both other dietary g
roups (P < 0.05 both cases), and the mixed-oil diet markedly increased
postprandial plasma insulin levels compared with the other dietary gr
oups (P < 0.001).