N-TERMINAL AMINO-ACIDS OF BRAZIL NUT 7S PROTEIN

Authors
CAI JS WELLER DL
Citation
Js. Cai et Dl. Weller, N-TERMINAL AMINO-ACIDS OF BRAZIL NUT 7S PROTEIN, Journal of food biochemistry, 19(1), 1995, pp. 43-49
Citations number
17
Categorie Soggetti
Biology,"Nutrition & Dietetics
Journal title
Journal of food biochemistry → ACNP
ISSN journal
01458884
Volume
19
Issue
1
Year of publication
1995
Pages
43 - 49
Database
ISI
SICI code
0145-8884(1995)19:1<43:NAOBN7>2.0.ZU;2-8
Abstract
N-terminal analysis of Brazil nut (Bertholettia excelsa) 7S protein wa s carried out using the dansyl chloride method and micellar electrokin etic capillary chromatography (MECC) for analysis of the dansyl-amino acids. Lysine, arginine and proline were identified as the N-terminal amino acids of the 7S protein. Subunits of the 7S protein, isolated by preparative SDS-PAGE, were also analyzed. The results are discussed i n terms of the subunit composition of the protein.