A RAPID METHOD FOR SEPARATION OF ANOMERIC SACCHARIDES USING A CYCLODEXTRIN-BONDED PHASE AND FOR INVESTIGATION OF MUTAROTATION

HPLC separation of anomeric sugars using a beta-cyclodextrin bonded ph ase and an eluent ethyl acetate/methanol/water has been achieved. HPLC is a useful method for analytically separating the anomers of sugars in a mutarotated system and is a good supplement to the nuclear magnet ic resonance. By means of this technique investigations of the mutarot ation of D-glucose gave 36% alpha-form and 64% beta-form. The pH depen dence of mutarotation in an organic solvent was examined and discussed . Further mono- and disaccharides were separated in anomers. Furanoid forms of the sugar can also be determined by HPLC.