DETERMINATION OF OCHRATOXIN-A IN CEREALS, MALT AND BEER AFTER ACCUMULATION AND SEPARATION ON IMMUNOAFFINITY COLUMNS AND FOLLOWING HIGH-PRESSURE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

This paper describes the combination of the clean-up and accumulation of ochratoxin A from different samples with immobilisied, specific ant i bodies and the high-pressure liquid chromatographic with fluorescenc e detection. The method was validated with barley as matrix. The resul t of the validation was, that the method is free of systematic errors. Barley, malt and beer were tested for ochratoxin A residues. 31% of t he barley samples were ochratoxin A positive (minimum 0,1 mu g/kg, max imum 2,7 mu g/kg) and 27% of the malt samples (minimum 0,1 mu g/kg, ma ximum 0,92 mu/kg), respectively. In beer ochratoxin A was found in the range from 0,03 to 0,08 mu g/kg.