The temperature and relative humidity of storage, as well as the gaseo
us environment, interact with the fertile egg over time during storage
in such a way as to affect the success of incubation either negativel
y or positively. This interaction occurs both above and below the ''ph
ysiological zero'', at which embryonic metabolism is minimal. This int
eraction below physiological zero implies that certain physical aspect
s of the egg must be affected by the environmental conditions. As the
eggshell is a relatively fixed component, changes in albumen, shell me
mbranes, cuticle, yolk, or embryo proper must account for these time-
and environment-related effects. It is concluded that the major contri
butor is the albumen, as it is obviously the most dynamic component be
low physiological zero and is strategically positioned.