C. Dinnella et G. Lanzarini, FRESH PLANT-TISSUE SOFTENING BY MEANS OF AN IMMOBILIZED SOLUBLE-INSOLUBLE ENDOPECTINLYASE, International journal of food science & technology, 30(3), 1995, pp. 391-396
An endopectinlyase (E.C. 4.2.2.10) immobilized on a reversible soluble
-insoluble polymer was used for macerating fresh carrot tissue. Tile m
acerating capability of the immobilized enzyme was found to be similar
to that of its free form. Losses of catalytic activity detected in th
e subsequent biocatalyst utilization cycles seem to be related to co-p
recipitation phenomena following the recovery of the immobilized enzym
e from the reaction mixture. Preliminary studies were carried out on t
he possibility of removing the inhibiting compounds and subsequently r
estoring biocatalyst activity.