CONSTRUCTION OF AN ENDOGLUCANOLYTIC BREWING YEAST-STRAIN

An endo-beta(1,4)glucanase encoding gene from fungal origin has been e xpressed in a brewing yeast strain. The yeast transformation was carri ed out using a previously reported system based in the acquisition of cycloheximide resistance. The plasmid transferred to brewing yeast sho wed some changes in restriction pattern of the 2 mu portion after tran sformation while cycloheximide resistance marker and endoglucanase gen e were not affected. The drug resistance phenotype showed by the recom binant yeast was highly stable in non-selective conditions. The endogl ucanase enzyme was detected in cell-free culture medium and showed hig h activities in liquid and solid media.