FACTORS AFFECTING THE ACYLTRANSFER ACTIVITY OF THE LIPASE FROM CANDIDA-PARAPSILOSIS IN AQUEOUS-MEDIA

This study describes the influence of various factors on the hydrolysi s and alcoholysis activities of the lipase from Candida parapsilosis ( Ashford), Langeron and Talice in aqueous media. Optimal activities wer e obtained at 45 degrees C and pH 6-6.5. The influence of the nature o f the substrate on the temperature activity profiles was observed. Tot al or partial recovery of the activities was obtained when methanol wa s added to the enzyme extract after thermal denaturation. A tyrosin re sidue appeared to be necessary for lipase function. Magnesium was a re quired metal cofactor. These activities were optimal in the presence o f high amounts of water (water activity > 0.9).