Samples (32) of natural milk cultures used in the Santa Fe, Argentina,
area for soft and semihard cheese production were examined. The micro
bial composition (including lactic acid microflora characterization) a
nd technological parameters (acidifying and proteolytic activities) we
re evaluated. The cultures contained mainly thermophilic lactic acid b
acteria, identified as Streptococcus salivarius subsp. thermophilus (9
6.8% of the total strains) and Enterococcus spp. The strains showed a
low proteolytic activity. The isolates of S. salivarius subsp. thermop
hilus exhibited a widespread phage resistance. The nonlactic microflor
a comprised coliforms, yeasts, spore-forming bacteria and lactate ferm
entative bacteria. The samples showed an acidity level from 0.38 to 0.
69% lactic acid (pH from 4.25 to 5.75). The acidifying activity was op
timal at 45 degrees C. The advantages and disadvantages of the employm
ent of natural milk starters are discussed.