DETECTION OF 2,4-DICHLOROPHENOXYACETIC AC ID IN LEMONS WITH AN ELISA KIT

The effect of methanol or acetone in 0.0 1 M phosphate-buffered saline (pH 7.2) at various concentrations on a commercially available 2,4-D detecting ELISA kit was investigated. The optical density in the ELISA reaction was unaffected by 0 approximately 30% methanol in the buffer solutions, while acetone decreased the optical density with increase of its concentration. Fungicides, such as o-phenylphenol or thiabendaz ol, did not disturb the ELISA reaction at the concentration of 20 ppm or 100 ppm, respectively. The 2,4-D in lemon peel was easily detected using 30% methanol in the buffer solution. The determination took 80 m in from the start of extraction.