Pj. Wilbrandt, A SIMPLE CONCEPT FOR BETTER ALIGNMENT AND SIMPLIFIED OPERATION OF A TRANSMISSION ELECTRON-MICROSCOPE, Ultramicroscopy, 52(2), 1993, pp. 193-204
The operation of transmission electron microscopes is still complicate
d despite of the numerous improvements in the past. Many adjustment st
eps are still necessary for the routine alignment and, above all, the
microscope has to be readjusted frequently during operation. This prob
lem is mainly caused by the dependence of the microscope alignment on
the objective lens excitation. It will be shown that the focus range f
or a good microscope alignment is limited to +/-50 mu m for CTEM and b
s +/-4 mu m for HREM. Yet larger focus variations are frequently neces
sary to compensate height variations of the specimen by tilting or by
shifting if the specimen is buckled. As solution of this problem it is
proposed to keep the objective lens current constant and to focus the
image mechanically by adjusting the specimen height. The practicabili
ty and consequences of this way of microscope operation are critically
assessed.