CARNOCIN UI49, A POTENTIAL BIOPRESERVATIVE PRODUCED BY CARNOBACTERIUM-PISCICOLA - LARGE-SCALE PURIFICATION AND ACTIVITY AGAINST VARIOUS GRAM-POSITIVE BACTERIA INCLUDING LISTERIA SP

This paper describes a simple purification method for the purification of carnocin UI49, a potential biopreservative produced by Carnobacter ium piscicola UI49. The protocol was also applicable for the isolation of nisin Z, which is a biopreservative produced by Lactococcus lactis SIK-83. The protocol consists of only two purification steps, XAD chr omatography and cation exchange chromatography. It is quick, easy, and can be used for large scale purification of these lantibiotics. The b actericidal activity of carnocin U149 against carnobacteria, lactococc i and Listeria was compared with that of nisin Z. The carnobacteria sh owed similar sensitivity towards carnocin UI49 and nisin. The nisin pr oducing L. lactis strains were very sensitive towards carnocin UI49, w hile the non-producing L. lactis strains were more sensitive to nisin. The Listeria strains were weakly sensitive to carnocin UI49, lower co ncentrations of nisin were needed to inhibit growth.